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重大慢性疾病-糖尿病-
AMPK失调引起糖尿病病患过氧化物减少和线粒体功能下降
上传日期:2014-01-14       共有1385人次浏览
 
 
题名: AMPK dysregulation promotes diabetes-related reduction of superoxide and mitochondrial function
作者: Laura L. Dugan, Young-Hyun You, Sameh S. Ali, et al.
单位: Division of Geriatrics, Department of Medicine, and Center for Renal Translational Medicine, Division of Nephrology-Hypertension, Department of Medicine, UCSD, La Jolla, California, USA.
出处: J Clin Invest,2013,123(11):4888–4899
语种: 英文
文摘:

 

Diabetic microvascular complications have been considered to be mediated by a glucose-driven increase in mitochondrial superoxide anion production. Here, we report that superoxide production was reduced in the kidneys of a steptozotocin-induced mouse model of type 1 diabetes, as assessed by in vivo real-time transcutaneous fluorescence, confocal microscopy, and electron paramagnetic resonance analysis. Reduction of mitochondrial biogenesis and phosphorylation of pyruvate dehydrogenase (PDH) were observed in kidneys from diabetic mice. These observations were consistent with an overall reduction of mitochondrial glucose oxidation. Activity of AMPK, the major energy-sensing enzyme, was reduced in kidneys from both diabetic mice and humans. Mitochondrial biogenesis, PDH activity, and mitochondrial complex activity were rescued by treatment with the AMPK activator 5-aminoimidazole-4-carboxamide-1-β-D-ribofuranoside (AICAR). AICAR treatment induced superoxide production and was linked with glomerular matrix and albuminuria reduction in the diabetic kidney. Furthermore, diabetic heterozygous superoxide dismutase 2 (Sod2+/–) mice had no evidence of increased renal disease, andAmpka2–/– mice had increased albuminuria that was not reduced with AICAR treatment. Reduction of mitochondrial superoxide production with rotenone was sufficient to reduce AMPK phosphorylation in mouse kidneys. Taken together, these results demonstrate that diabetic kidneys have reduced superoxide and mitochondrial biogenesis and activation of AMPK enhances superoxide production and mitochondrial function while reducing disease activity.

 

http://dx.doi.org/10.1172/JCI66218

关键词: AMPK dysregulation; diabetes; superoxide; mitochondrial

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